ExperimentSetReplicate

ExperimentSet4DNES7N27U89
released
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In situ Hi-C with DpnII on HeLa-S3 (non synchronized) with 1% Formaldehyde and 3mM EGS crosslinking

   June 27th, 2020 at 6:56pm
Source Publication
Systematic evaluation of chromosome conformation capture assays.
Akgol Oksuz B, Yang L, et al., Nature methods 2021

WARNING: non-optimal cross-linking conditions  

This dataset may be obtained with non-optimal cross-linking conditions (as demonstrated in the paper) and is reported here for documentation purposes.

Users interested in using Hi-C or Micro-C data from this project for alternative analyses are advised to use only datasets generated with the optimal cross-linking conditions of 1% Formaldehyde + 3mM DSG. The study authors further advise users to carefully select digestion enzymes and library size to fit their needs. A list of preferred datasets for re-use is provided.

Assay Description  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
HeLa-S3
Experiment Type(s) 
in situ Hi-C
Modification Type 
None
Treatment Type 
None
Assay Details 
Enzyme: DpnII

8 Processed Files

Quality Metrics

Experiment
For File
Filtered Reads
Cis reads (>20kb)
Short cis reads
Trans Reads
Report
Details
From Multiple Experiments
4DNES7N27U89
Multiple Experiments
File
Contact List-combined (pairs)
4DNFIHRT8ASR
122.61m
46.253%
35.038%
18.709%
Experiment
4DNEX4QN19KR
Bio Rep 1, Tec Rep 1
in situ Hi-C on HeLa-S3 with DpnII
File
Contact List-replicate (pairs)
4DNFIRIGR1GB
122.61m
46.253%
35.038%
18.709%
Experiment
For File
Failed Balancing
Details
From Multiple Experiments
4DNES7N27U89
Multiple Experiments
File
Contact Matrix (mcool)
4DNFI2QRPR77
None
Experiment
For File
Total Reads
Unmapped Reads
Multimapped Reads
Duplicate Reads
Walks
Minor Contigs
Details
Experiment
4DNEX4QN19KR
Bio Rep 1, Tec Rep 1
in situ Hi-C on HeLa-S3 with DpnII
File
Alignments (bam)
4DNFI289MKFY
362.53m
18.033%
21.961%
26.18%
0.006%
0.044%