ExperimentSetReplicate

ExperimentSet4DNESE2MH977
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Multiplexed FISH in Sox2 tagged mouse embryonic stem cell line (F123-CASTx129) with a 7.5kb insertion containing 4 CTCF binding sites introduced between Sox2 and its Super Enhancer, treated with A485 to inhibit p300 activity for 12 hrs followed with 6hr recovery from A485 treatment.

   February 3rd, 2025 at 3:47pm

Experiment Set Properties  

Set Type 
Replicate
Organism 
M. musculus
Sample Type 
stem cells
Sample 
F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE
Experiment Type(s) 
multiplexed FISH
Modification Type 
Crispr
Treatment Type 
Chemical
Assay Details 
Target: 101 40-bp regions on Chr11 (mouse), 4470 40-bp on the 205kb stretch from Sox2 gene to SE and surrounding regions (mouse), Chromosomes
Channel
Imaging Rounds
Path
 
ch00
1
DAPI staining
ch03
1
101 40-bp regions on Chr11 (mouse) placed as a 7.5kb insertion between Sox2 promoter and SE, targeted by Alexa Fluor 647 (Imaging probes)
ch01
2-15
4470 40-bp on the 205kb stretch from Sox2 gene to SE and surrounding regions (mouse) targeted by Fluospheres 505 (Fiducial probes)
ch02
2-15
4470 40-bp on the 205kb stretch from Sox2 gene to SE and surrounding regions (mouse) targeted by Alexa Fluor 750 (Imaging probes)
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1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEXYPSCL48
Bio Rep 1, Tec Rep 1
multiplexed FISH on F123-CASTx129 with Sox2 tags and RMCE site between Sox2 and its SE
File
4DNFIP1EKJ7Z
FOF-CT - DNA-spot/trace core (csv)
22.59 MB