{"lab": {"uuid": "3b4ae9a9-e440-4918-8923-38bec007a650", "@id": "/labs/robert-coleman-lab/", "title": "Robert Coleman, EINSTEIN", "status": "current", "correspondence": [{"contact_email": "cm9iZXJ0LmNvbGVtYW4yQGVpbnN0ZWluLnl1LmVkdQ==", "@id": "/users/ac1542b9-f3de-491f-9f2d-f9f0be5c0f56/", "display_title": "Robert Coleman"}], "display_title": "Robert Coleman, EINSTEIN", "@type": ["Lab", "Item"], "pi": {"error": "no view permissions"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.3b4ae9a9-e440-4918-8923-38bec007a650"]}}, "award": {"@id": "/awards/1U01EB021236-01/", "display_title": "TOOLS FOR IMAGING THE FUNCTIONAL GENOME IN LIVING CELLS AND TISSUES", "status": "current", "center_title": "IT - Singer", "project": "4DN", "description": "IT: This goal of this proposal is to develop imaging tools capable of imaging the functional genome by mapping the three dimensional binding sites and clustering of transcription factors and histone modifiers. 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SPT

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Single particle tracking (SPT) is a method to track the position\n of a single molecule over time in live cells. Advances in \nmicroscopy and molecular biology techniques have enabled a \nbroad range of applications.

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The protocol involves preparing the target molecule to be \nidentifiable under the microscope, tracking its position over \ntime and analyzing its trajectory. Generally, the preparation \nof the molecule of interest is performed by genetically \nmodifying it with tags followed by the binding of reporter \nmolecules such as fluorescent dyes. However, depending on the \nmolecule of interest, the goal of the experiment and the type \nof microscope used, different techniques are also implemented.\n Following the preparation of the molecule, a high-resolution \nmicroscope is used to take snapshots of the position of the \nmolecule at different intervals of time. Tracking a single \nmolecule in a live cell is a demanding task, requiring both \nsensitivity and accuracy. Tailored to the application, this \ncan be achieved by specialized microscopy techniques like TIRF\n or PALM. Analysis of the resulting images results in a file \nthat describes the trajectory of the molecule.

\n

4DN processed files are in a format that includes the ID of a \ntrajectory, the time interval, and the coordinates of the \nposition. Further analysis of the trajectory of the molecule \nis necessary to gain biological insight.

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