ExperimentSetReplicate

ExperimentSet4DNESMWANQOC
released
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Validation experiment of fast 3D, two-color single-molecule switching nanoscopy on relatively thin targets. This sample targets one structure (mitochondrial nucleoids) which is expected to be inside of the organelle targeted by the other probe (mitochondrial outer membrane).

   August 5th, 2019 at 3:32pm
Source Publication
An integrated platform for high-throughput nanoscopy.
Barentine AES, Lin Y, et al., Nature biotechnology 2023

Note on Localization h5 Processed Files  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
immortalized cells
Sample 
U2OS (Tier 2)
Experiment Type(s) 
Immunofluorescence
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: TOMM20 protein, Chromosomes
Sample Image 
Sample rendered image of mitochondrial nucleoids - TOMM20 (red) and DNA (green)
View File Item - 4DNFIU7ODDMZ
Channel
Path
 
ch00
TOMM20 protein targeted by anti-TOMM20 (with anti-Rabbit, Alexa Fluor 647)
ch01
Chromosomes targeted by anti-ds DNA (with anti-Mouse, CF568)

1 Processed File

Experiment
File
File Type
File Size
Notes
Experiment
4DNEXHV5ZRHE
Bio Rep 1, Tec Rep 1
Immunofluorescence on U2OS (Tier 2)
File
4DNFIMCSA8UD
localizations (hdf5)
6.45 MB