ExperimentSetReplicate

ExperimentSet4DNESPADIUQ8
released
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Chromatin tracing at chromosome X H9 cells in all naive culture conditions combined with XIST RNA FISH (XIST+ chromosomes)

   August 22nd, 2023 at 7:15pm
Source Publication
Female naive human pluripotent stem cells carry X chromosomes with Xa-like and Xi-like folding conformations.
Patterson B, Yang B, et al., Science advances 2023

Note on FOF-CT file format  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
stem cells
Sample 
H9
Experiment Type(s) 
multiplexed FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: 20 TADs chrX (1-20), 20 TADs chrX (21-40), XIST transcript
Channel
Imaging Rounds
Path
 
ch00
1-20
Alexa Fluor 488-labeled fiducial beads
ch02
1-20
20 TADs chrX (1-20) targeted by Alexa Fluor 647-labeled imaging oligos
ch03
1-20
20 TADs chrX (21-40) targeted by ATTO 565-labeled imaging oligos
ch01
-
XIST transcript targeted by Cy3-labeled imaging oligos

1 Processed File

Experiment
File
File Type
File Size
Experiment
4DNEXRXUBJM7
Bio Rep 1, Tec Rep 1
multiplexed FISH on H9
File
4DNFIPNWMI3V
FOF-CT - DNA-spot/trace core (csv)
410.12 kB