{"lab": {"title": "Wei Xie, TSINGHUA", "status": "current", "@type": ["Lab", "Item"], "uuid": "8c95312f-afa0-49ee-9d3c-e30849c74e50", "display_title": "Wei Xie, TSINGHUA", "@id": "/labs/wei-xie-lab/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.8c95312f-afa0-49ee-9d3c-e30849c74e50"]}}, "award": {"@id": "/awards/external-award/", "description": "Funding source is from outside 4DN.", "uuid": "12a92962-8265-4fc0-b2f8-cf14f05db58b", "name": "external-award", "display_title": "EXTERNAL AWARD", "center_title": "External", "project": "External", "@type": ["Award", "Item"], "status": "current", "center": "External", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "study": "Embryo Development", "status": "released", "aliases": ["4dn-dcic-lab:sisHi-C_alpha-amanitin_20h"], "accession": "4DNESTHQ7CR1", "condition": "In vitro for 20 h with alpha-amanitin (100 \u03bcg/ml)", "description": "Small-scale in situ Hi-C on mouse preimplantation embryos collected at PN3 zygotic stage and cultured in vitro for 20 h with alpha-amanitin", "study_group": "Time Course", "date_created": "2020-01-10T15:45:05.185459+00:00", "submitted_by": {"error": "no view permissions"}, "dataset_group": "sisHi-C during early embryonic development", "dataset_label": "sisHi-C of early embryos in vitro", "last_modified": {"modified_by": {"error": "no view permissions"}, "date_modified": "2024-07-31T14:46:29.106921+00:00"}, "public_release": "2020-02-07", "replicate_exps": [{"bio_rep_no": 1, "tec_rep_no": 1, "replicate_exp": {"uuid": "38b1ee43-440e-40e5-a360-9696b663e8e7", "status": "released", "@id": "/experiments-hi-c/4DNEXJFZUVEK/", "@type": ["ExperimentHiC", "Experiment", "Item"], "display_title": "in situ Hi-C on embryo with MboI - 4DNEXJFZUVEK", "accession": "4DNEXJFZUVEK", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}, {"bio_rep_no": 2, "tec_rep_no": 1, "replicate_exp": {"uuid": "306a7111-b4c5-44f0-b77d-23ccc7f9a2fb", "status": "released", "@id": "/experiments-hi-c/4DNEXGABZ8RE/", "@type": ["ExperimentHiC", "Experiment", "Item"], "display_title": "in situ Hi-C on embryo with MboI - 4DNEXGABZ8RE", "accession": "4DNEXGABZ8RE", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "schema_version": "2", "static_content": [{"content": {"filetype": "HiglassViewConfig", "@id": "/higlass-view-configs/d4992af5-4fac-478e-9e0e-53f5a7f74715/", "@type": ["HiglassViewConfig", "UserContent", "Item"], "status": "released", "contributing_labs": [], "uuid": "d4992af5-4fac-478e-9e0e-53f5a7f74715", "award": {"status": "current", "display_title": "EXTERNAL AWARD", "@id": "/awards/external-award/", "uuid": "12a92962-8265-4fc0-b2f8-cf14f05db58b", "@type": ["Award", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "description": "4DNESTHQ7CR1 (Small-scale in situ Hi-C on mouse preimplantation embryos collected at PN3 zygotic stage and cultured in vitro for 20 h with alpha-amanitin): 4DNFI7875Q16, 4DNFITLHAFZC", "title": "4DNESTHQ7CR1 - Processed files", "name": "d4992af5-4fac-478e-9e0e-53f5a7f74715", "display_title": "4DNESTHQ7CR1 - Processed files", "lab": {"@id": "/labs/wei-xie-lab/", "status": "current", "@type": ["Lab", "Item"], "display_title": "Wei Xie, TSINGHUA", "uuid": "8c95312f-afa0-49ee-9d3c-e30849c74e50", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.8c95312f-afa0-49ee-9d3c-e30849c74e50"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.owner", "userid.7677f8a8-79d2-4cff-ab0a-a967a2a68e39"]}}, "location": "tab:processed-files", "description": "auto_generated_higlass_view_config"}], "static_headers": [{"lab": {"display_title": "4DN DCIC, HMS", "uuid": "828cd4fe-ebb0-4b36-a94a-d2e3a36cc989", "@type": ["Lab", "Item"], "status": "current", "@id": "/labs/4dn-dcic-lab/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.828cd4fe-ebb0-4b36-a94a-d2e3a36cc989"]}}, "body": "<b>In Situ Hi-C</b>\n\n<p>\n   In situ Hi-C is a method to detect and quantify the pairwise interactions between chromosome regions across the entire genome. It was developed in 2014 as an improvement over the dilution Hi-C method. Compared to standard dilution Hi-C, this technique reduces the frequency of random ligation because the ligation is performed <i>in situ</i> inside the nucleus, a constrained space, instead of in solution, where DNA fragments are floating freely. In addition, this protocol can be done more quickly in the lab and was the first to introduce the use of 4-cutter restriction enzymes as opposed to the previous 6-cutters, providing higher resolution.\n</p>\n<p>\nThe protocol involves cross-linking the cells with formaldehyde to form links between physically adjacent DNA regions. The cells are then permeabilized with their nuclei intact. A 4-cutter restriction enzyme is used to digest the chromatin into multiple DNA fragments. The resulting fragments are biotinylated by end filling of the fragments ends. The fragments are then ligated and the DNA is purified and sheared. The biotinylated fragments are pulled down from the solution with streptavidin beads and a library is constructed and sequenced. Analysis of the resulting paired-end short read sequences produces a matrix that shows the number of interactions between different DNA regions.\n</p>\n<p>\nSee <a href=\"https://www.sciencedirect.com/science/article/pii/S0092867414014974?via%3Dihub\">Rao et al., 2014</a> for more details.\n</p>\n\n<div>\n<img style=\"width: 600px;max-width:100%;\" src=\"https://s3.amazonaws.com/4dn-dcic-public/static-pages/InfoBoxes/IsHC_fig1.png\"/>\n  <br/><br/>\n  <em>Image source: Rao et al., 2014, Figure 1A</em>\n</div>", "name": "item-page-headers.ExperimentType.insituhic", "award": {"display_title": "4D NUCLEOME NETWORK DATA COORDINATION AND INTEGRATION CENTER - PHASE I", "status": "current", "uuid": "b0b9c607-f8b4-4f02-93f4-9895b461334b", "@type": ["Award", "Item"], "@id": "/awards/1U01CA200059-01/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "title": "Assay Description", "status": "released", "aliases": ["4dn-dcic-lab:experiment_infobox_hic"], "options": {"filetype": "html", "collapsible": false, "default_open": false, "convert_ext_links": true}, "date_created": "2018-09-07T18:19:43.777198+00:00", "section_type": "Item Page Header", "submitted_by": {"error": "no view permissions"}, "last_modified": {"modified_by": {"error": "no view permissions"}, "date_modified": "2023-11-09T14:48:01.220007+00:00"}, "schema_version": "2", "@id": "/static-sections/298554ad-20e2-4449-a752-ac190123dab7/", "@type": ["StaticSection", "UserContent", "Item"], "uuid": "298554ad-20e2-4449-a752-ac190123dab7", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.owner", "userid.56c9c683-bb11-471b-b590-c656f7dc03c1"]}, "display_title": "Assay Description", "external_references": [], "content": "<b>In Situ Hi-C</b>\n\n<p>\n   In situ Hi-C is a method to detect and quantify the pairwise interactions between chromosome regions across the entire genome. It was developed in 2014 as an improvement over the dilution Hi-C method. Compared to standard dilution Hi-C, this technique reduces the frequency of random ligation because the ligation is performed <i>in situ</i> inside the nucleus, a constrained space, instead of in solution, where DNA fragments are floating freely. In addition, this protocol can be done more quickly in the lab and was the first to introduce the use of 4-cutter restriction enzymes as opposed to the previous 6-cutters, providing higher resolution.\n</p>\n<p>\nThe protocol involves cross-linking the cells with formaldehyde to form links between physically adjacent DNA regions. The cells are then permeabilized with their nuclei intact. A 4-cutter restriction enzyme is used to digest the chromatin into multiple DNA fragments. The resulting fragments are biotinylated by end filling of the fragments ends. The fragments are then ligated and the DNA is purified and sheared. The biotinylated fragments are pulled down from the solution with streptavidin beads and a library is constructed and sequenced. Analysis of the resulting paired-end short read sequences produces a matrix that shows the number of interactions between different DNA regions.\n</p>\n<p>\nSee <a href=\"https://www.sciencedirect.com/science/article/pii/S0092867414014974?via%3Dihub\">Rao et al., 2014</a> for more details.\n</p>\n\n<div>\n<img style=\"width: 600px;max-width:100%;\" src=\"https://s3.amazonaws.com/4dn-dcic-public/static-pages/InfoBoxes/IsHC_fig1.png\"/>\n  <br/><br/>\n  <em>Image source: Rao et al., 2014, Figure 1A</em>\n</div>", "filetype": "html", "content_as_html": "<div class=\"html-container\"><b>In Situ Hi-C</b>\n<p>\n   In situ Hi-C is a method to detect and quantify the pairwise interactions between chromosome regions across the entire genome. It was developed in 2014 as an improvement over the dilution Hi-C method. Compared to standard dilution Hi-C, this technique reduces the frequency of random ligation because the ligation is performed <i>in situ</i> inside the nucleus, a constrained space, instead of in solution, where DNA fragments are floating freely. In addition, this protocol can be done more quickly in the lab and was the first to introduce the use of 4-cutter restriction enzymes as opposed to the previous 6-cutters, providing higher resolution.\n</p>\n<p>\nThe protocol involves cross-linking the cells with formaldehyde to form links between physically adjacent DNA regions. The cells are then permeabilized with their nuclei intact. A 4-cutter restriction enzyme is used to digest the chromatin into multiple DNA fragments. The resulting fragments are biotinylated by end filling of the fragments ends. The fragments are then ligated and the DNA is purified and sheared. The biotinylated fragments are pulled down from the solution with streptavidin beads and a library is constructed and sequenced. Analysis of the resulting paired-end short read sequences produces a matrix that shows the number of interactions between different DNA regions.\n</p>\n<p>\nSee <a href=\"https://www.sciencedirect.com/science/article/pii/S0092867414014974?via%3Dihub\" rel=\"noopener noreferrer\" target=\"_blank\">Rao et al., 2014</a> for more details.\n</p>\n<div>\n<img src=\"https://s3.amazonaws.com/4dn-dcic-public/static-pages/InfoBoxes/IsHC_fig1.png\" style=\"width: 600px;max-width:100%;\"/>\n<br/><br/>\n<em>Image source: Rao et al., 2014, Figure 1A</em>\n</div></div>"}, {"lab": {"display_title": "4DN DCIC, HMS", "uuid": "828cd4fe-ebb0-4b36-a94a-d2e3a36cc989", "@type": ["Lab", "Item"], "status": "current", "@id": "/labs/4dn-dcic-lab/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.828cd4fe-ebb0-4b36-a94a-d2e3a36cc989"]}}, "body": "Some annotated bam and pairs files generated in this Experiment Set may contain distinct reads that share the same identifier. This was caused by merging fastq files with integer identifiers (eg. @1, @2) during processing.", "name": "item-page-header.ES_files_with_integer_id_issue_warning_hic", "award": {"display_title": "4D NUCLEOME NETWORK DATA COORDINATION AND INTEGRATION CENTER - PHASE I", "status": "current", "uuid": "b0b9c607-f8b4-4f02-93f4-9895b461334b", "@type": ["Award", "Item"], "@id": "/awards/1U01CA200059-01/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "title": "Note on Processed Files", "status": "released", "aliases": ["4dn-dcic-lab:files_with_integer_id_issue_warning_hic_ES"], "options": {"filetype": "md", "collapsible": true, "default_open": true}, "date_created": "2020-04-06T19:36:49.278997+00:00", "section_type": "Item Page Header", "submitted_by": {"error": "no view permissions"}, "last_modified": {"modified_by": {"error": "no view permissions"}, "date_modified": "2020-04-19T23:58:37.106537+00:00"}, "schema_version": "2", "@id": "/static-sections/a16d9553-7158-4121-80f7-d1342213b3fa/", "@type": ["StaticSection", "UserContent", "Item"], "uuid": "a16d9553-7158-4121-80f7-d1342213b3fa", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.owner", "userid.56c9c683-bb11-471b-b590-c656f7dc03c1"]}, "display_title": "Note on Processed Files", "external_references": [], "content": "Some annotated bam and pairs files generated in this Experiment Set may contain distinct reads that share the same identifier. This was caused by merging fastq files with integer identifiers (eg. @1, @2) during processing.", "filetype": "md", "content_as_html": "<div class=\"markdown-container\"><p>Some annotated bam and pairs files generated in this Experiment Set may contain distinct reads that share the same identifier. This was caused by merging fastq files with integer identifiers (eg. @1, @2) during processing.</p></div>"}], "processed_files": [{"@id": "/files-processed/4DNFITDL2BAJ/", "accession": "4DNFITDL2BAJ", "genome_assembly": "GRCm38", "uuid": "d320749f-1253-4fc3-b285-e26d3bdc607a", "file_type_detailed": "contact list-combined (pairs)", "file_classification": "processed file", "md5sum": "3995704d25ab8812852c7acb1eded5df", "@type": ["FileProcessed", "File", "Item"], "file_size": 955196005, "display_title": "4DNFITDL2BAJ.pairs.gz", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/wfoutput/d320749f-1253-4fc3-b285-e26d3bdc607a/4DNFITDL2BAJ.pairs.gz", "href": "/files-processed/4DNFITDL2BAJ/@@download/4DNFITDL2BAJ.pairs.gz", "status": "released", "file_format": {"@type": ["FileFormat", "Item"], "display_title": "pairs", "uuid": "d13d06cf-218e-4f61-aaf0-91f226248b2c", "status": "released", "@id": "/file-formats/pairs/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "notes_to_tsv": ["WARNING: some distinct reads in this file may share the same identifier because the file was generated after merging fastq files with integer identifiers (eg. @1, @2) during processing."], "upload_key": "d320749f-1253-4fc3-b285-e26d3bdc607a/4DNFITDL2BAJ.pairs.gz", "file_type": "contact list-combined", "last_modified": {"date_modified": "2021-04-06T20:18:57.420589+00:00"}, "lab": {"status": "current", "display_title": "Wei Xie, TSINGHUA", "name": "wei-xie-lab", "uuid": "8c95312f-afa0-49ee-9d3c-e30849c74e50", "@type": ["Lab", "Item"], "@id": "/labs/wei-xie-lab/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.8c95312f-afa0-49ee-9d3c-e30849c74e50"]}}, "quality_metric": {"url": "https://s3.amazonaws.com/elasticbeanstalk-fourfront-webprod-wfoutput/4DNFITDL2BAJ/pairsqc_report.html", "@id": "/quality-metrics-pairsqc/be1e910e-c425-464d-a7fb-3867b5f5310f/", "@type": ["QualityMetricPairsqc", "QualityMetric", "Item"], "Total reads": 65279070, "overall_quality_status": "PASS", "uuid": "be1e910e-c425-464d-a7fb-3867b5f5310f", "display_title": "QualityMetricPairsqc from 2020-01-24", "status": "released", "quality_metric_summary": [{"title": "Filtered Reads", "value": "65279070", "numberType": "integer"}, {"title": "Cis reads (>20kb)", "value": "54.115", "tooltip": "Percent of filtered reads (=35.33m)", "numberType": "percent"}, {"title": "Short cis reads", "value": "27.625", "tooltip": "Percent of filtered reads (=18.03m)", "numberType": "percent"}, {"title": "Trans Reads", "value": "18.26", "tooltip": "Percent of filtered reads (=11.92m)", "numberType": "percent"}], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "track_and_facet_info": {"dataset": "sisHi-C of early embryos in vitro", "condition": "In vitro for 20 h with alpha-amanitin (100 \u03bcg/ml)", "experimental_lab": "Wei Xie, TSINGHUA", "replicate_info": "merged 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"752381c550194f6ebfa3b6d61dccb75b", "@type": ["FileProcessed", "File", "Item"], "file_size": 1030550206, "display_title": "4DNFIQZRKS7Y.hic", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/wfoutput/ce9e9334-ee0b-4549-a845-110470ba5986/4DNFIQZRKS7Y.hic", "href": "/files-processed/4DNFIQZRKS7Y/@@download/4DNFIQZRKS7Y.hic", "status": "released", "file_format": {"@type": ["FileFormat", "Item"], "display_title": "hic", "uuid": "d13d11cf-218e-4f61-bbf0-91f226248b2c", "status": "released", "@id": "/file-formats/hic/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "upload_key": "ce9e9334-ee0b-4549-a845-110470ba5986/4DNFIQZRKS7Y.hic", "file_type": "contact matrix", "last_modified": {"date_modified": "2020-04-14T02:39:41.205407+00:00"}, "lab": {"status": "current", "display_title": "Wei Xie, TSINGHUA", "name": "wei-xie-lab", "uuid": "8c95312f-afa0-49ee-9d3c-e30849c74e50", "@type": ["Lab", "Item"], "@id": "/labs/wei-xie-lab/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.8c95312f-afa0-49ee-9d3c-e30849c74e50"]}}, "track_and_facet_info": {"dataset": "sisHi-C of early embryos in vitro", "condition": "In vitro for 20 h with alpha-amanitin (100 \u03bcg/ml)", "experimental_lab": "Wei Xie, TSINGHUA", "replicate_info": "merged replicates", "experiment_bucket": "processed file", "experiment_type": "in situ Hi-C", "assay_info": "MboI", "biosource_name": "embryo", "lab_name": "Wei Xie, TSINGHUA"}, "external_references": [], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, {"@id": "/files-processed/4DNFI7875Q16/", "accession": "4DNFI7875Q16", "genome_assembly": "GRCm38", "uuid": "84f54fe9-448a-4ce9-931e-a97575f65d48", "file_type_detailed": "contact matrix (mcool)", "file_classification": "processed file", "higlass_uid": "3417ed7e-7168-447d-a628-3c60a83aba49", "md5sum": "636b97324d6ed97a32a1ad4a40aa67ba", "@type": ["FileProcessed", "File", "Item"], "file_size": 639510173, "display_title": "4DNFI7875Q16.mcool", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/wfoutput/84f54fe9-448a-4ce9-931e-a97575f65d48/4DNFI7875Q16.mcool", "href": "/files-processed/4DNFI7875Q16/@@download/4DNFI7875Q16.mcool", "status": "released", "file_format": {"@type": ["FileFormat", "Item"], "display_title": "mcool", "uuid": "d13d06cf-218e-4f61-ccf0-91f226248b2c", "status": "released", "@id": "/file-formats/mcool/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "notes_to_tsv": ["WARNING - Due to a bug in the version of cooler (0.8.3) used in the current 4DN standard Hi-C processing pipeline some pixels may occur mulitple times at a single resolution with different counts being reported for each occurence.  This duplication does not affect the higlass display of these files, howevver, downstream analyses using this file may encounter issues due to this pixel duplication.  The counts from the duplicate pixels can be aggregated to determine the correct count value at that location. If this issue is problematic for your needs you should consider regenerating the matrices from the merged pairs file of the associated dataset using a more recent version of cooler.  We are working to update the pipeline but do not yet have a predicted date for when this issue will be resolved."], "upload_key": "84f54fe9-448a-4ce9-931e-a97575f65d48/4DNFI7875Q16.mcool", "file_type": "contact matrix", "static_content": [{"description": "auto_generated_higlass_view_config", "location": "tab:higlass", "content": {"uuid": "97174488-f337-4395-a042-a948d9bd7adf", "status": "released", "@type": ["HiglassViewConfig", "UserContent", "Item"], "display_title": "4DNFI7875Q16 - contact matrix for embryo in situ Hi-C MboI", "@id": "/higlass-view-configs/97174488-f337-4395-a042-a948d9bd7adf/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.owner", "userid.7677f8a8-79d2-4cff-ab0a-a967a2a68e39"]}}}], "last_modified": {"date_modified": "2023-08-25T17:50:41.451483+00:00"}, "lab": {"status": "current", "display_title": "Wei Xie, TSINGHUA", "name": "wei-xie-lab", "uuid": "8c95312f-afa0-49ee-9d3c-e30849c74e50", "@type": ["Lab", "Item"], "@id": 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Another notable difference is that a filter is applied to remove reads with MAPQ scores below 30 prior to mcool file generation.", "higlass_view_config": {"uuid": "f37c6c02-37e5-4552-b23b-d3065a4ae2a9", "@id": "/higlass-view-configs/f37c6c02-37e5-4552-b23b-d3065a4ae2a9/", "description": "Supplementary Files (HiC Processing Pipeline - v0.3.0) for 4DNESTHQ7CR1 (Small-scale in situ Hi-C on mouse preimplantation embryos collected at PN3 zygotic stage and cultured in vitro for 20 h with alpha-amanitin): 4DNFIISIDT7V", "status": "released", "display_title": "4DNESTHQ7CR1 - HiC Processing Pipeline - v0.3.0", "@type": ["HiglassViewConfig", "UserContent", "Item"], "last_modified": {"date_modified": "2024-07-31T21:01:21.663288+00:00"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.owner", "userid.7677f8a8-79d2-4cff-ab0a-a967a2a68e39"]}}}], "@id": "/experiment-set-replicates/4DNESTHQ7CR1/", "@type": ["ExperimentSetReplicate", "ExperimentSet", "Item"], "uuid": "e8dd95f2-1c53-421f-a5a8-d635155a6060", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "display_title": "4DNESTHQ7CR1", "external_references": [], "produced_in_pub": {"@id": "/publications/7b570ea3-1ca6-4076-83a3-666d35819cf1/", "date_published": "2017-07-12", "@type": ["Publication", "Item"], "abstract": "In mammals, chromatin organization undergoes drastic reprogramming after fertilization. However, the three-dimensional structure of chromatin and its reprogramming in preimplantation development remain poorly understood. Here, by developing a low-input Hi-C (genome-wide chromosome conformation capture) approach, we examined the reprogramming of chromatin organization during early development in mice. We found that oocytes in metaphase II show homogeneous chromatin folding that lacks detectable topologically associating domains (TADs)  and chromatin compartments. Strikingly, chromatin shows greatly diminished higher-order structure after fertilization. Unexpectedly, the subsequent establishment of chromatin organization is a prolonged process that extends through preimplantation development, as characterized by slow consolidation of TADs and segregation of chromatin compartments. The two sets of parental chromosomes are spatially separated from each other and display distinct compartmentalization in zygotes. Such allele separation and allelic compartmentalization can be found as late as the 8-cell stage. Finally, we show that chromatin compaction in preimplantation embryos can partially proceed in the absence of zygotic transcription and is a multi-level hierarchical process. Taken together, our data suggest that chromatin may exist in a markedly relaxed state after fertilization, followed by progressive maturation of higher-order chromatin architecture during early development.", "title": "Allelic reprogramming of 3D chromatin architecture during early mammalian development.", "status": "current", "journal": "Nature", "authors": ["Du Z", "Zheng H", "Huang B", "Ma R", "Wu J", "Zhang X", "He J", "Xiang Y", "Wang Q", "Li Y", "Ma J", "Zhang X", "Zhang K", "Wang Y", "Zhang MQ", "Gao J", "Dixon JR", "Wang X", "Zeng J", "Xie W"], "uuid": "7b570ea3-1ca6-4076-83a3-666d35819cf1", "short_attribution": "Du Z et al. (2017)", "display_title": "Du Z et al. (2017) PMID:28703188", "ID": "PMID:28703188", "url": "https://www.ncbi.nlm.nih.gov/pubmed/28703188", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "pubs_using": [], "publications_of_set": [{"ID": "PMID:28703188", "uuid": "7b570ea3-1ca6-4076-83a3-666d35819cf1", "date_published": "2017-07-12", "@type": ["Publication", "Item"], "abstract": "In mammals, chromatin organization undergoes drastic reprogramming after fertilization. However, the three-dimensional structure of chromatin and its reprogramming in preimplantation development remain poorly understood. Here, by developing a low-input Hi-C (genome-wide chromosome conformation capture) approach, we examined the reprogramming of chromatin organization during early development in mice. We found that oocytes in metaphase II show homogeneous chromatin folding that lacks detectable topologically associating domains (TADs)  and chromatin compartments. Strikingly, chromatin shows greatly diminished higher-order structure after fertilization. Unexpectedly, the subsequent establishment of chromatin organization is a prolonged process that extends through preimplantation development, as characterized by slow consolidation of TADs and segregation of chromatin compartments. The two sets of parental chromosomes are spatially separated from each other and display distinct compartmentalization in zygotes. Such allele separation and allelic compartmentalization can be found as late as the 8-cell stage. Finally, we show that chromatin compaction in preimplantation embryos can partially proceed in the absence of zygotic transcription and is a multi-level hierarchical process. Taken together, our data suggest that chromatin may exist in a markedly relaxed state after fertilization, followed by progressive maturation of higher-order chromatin architecture during early development.", "journal": "Nature", "title": "Allelic reprogramming of 3D chromatin architecture during early mammalian development.", "@id": "/publications/7b570ea3-1ca6-4076-83a3-666d35819cf1/", "display_title": "Du Z et al. (2017) PMID:28703188", "authors": ["Du Z", "Zheng H", "Huang B", "Ma R", "Wu J", "Zhang X", "He J", "Xiang Y", "Wang Q", "Li Y", "Ma J", "Zhang X", "Zhang K", "Wang Y", "Zhang MQ", "Gao J", "Dixon JR", "Wang X", "Zeng J", "Xie W"], "status": "current", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "number_of_experiments": 2, "@context": "/terms/", "aggregated-items": {"badges": []}, "validation-errors": []}