ExperimentSetReplicate

ExperimentSet4DNESWKPWJD5
released
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Validation experiment of fast 3D, two-color single-molecule switching nanoscopy on a volume the size of the nucleus. Test of imaging two FISH-labeled targets simultaneously.

   August 5th, 2019 at 3:32pm
Source Publication
An integrated platform for high-throughput nanoscopy.
Barentine AES, Lin Y, et al., Nature biotechnology 2023

Assay Description  

Note on Localization h5 Processed Files  

Experiment Set Properties  

Set Type 
Replicate
Organism 
H. sapiens
Sample Type 
primary cells
Sample 
IMR-90 (Tier 1)
Experiment Type(s) 
DNA FISH
Modification Type 
None
Treatment Type 
None
Assay Details 
Target: GRCh38:5:116144601-116449681 region, Chromosome 22 TADs
Sample Image 
Sample rendered image - FISH of a chromosome 5 LAD (green) and chromosome 22 TADs (red)
View File Item - 4DNFISK3CGUX
Channel
Path
 
ch00
GRCh38:5:116144601-116449681 region targeted by Alexa Fluor 647
ch01
Chromosome 22 TADs targeted by anti-Biotin (with anti-Mouse, CF568)

1 Processed File

Experiment
File
File Type
File Size
Notes
Experiment
4DNEX3M9UD4G
Bio Rep 1, Tec Rep 1
DNA FISH on IMR-90 (Tier 1)
File
4DNFIMR2OS49
localizations (hdf5)
1.88 MB