ExperimentSetReplicate

ExperimentSet4DNESZGE3UM2
released

In situ Hi-C with HindIII on HeLa-S3 (mitotic) with 1% Formaldehyde and 3mM EGS crosslinking

   June 27th, 2020 at 6:56pm
Source Publication
Systematic evaluation of chromosome conformation capture assays.
Akgol Oksuz B, Yang L, et al., Nature methods 2021

WARNING: non-optimal cross-linking conditions  


This dataset may be obtained with non-optimal cross-linking conditions (as demonstrated in the paper) and is reported here for documentation purposes.

Users interested in using Hi-C or Micro-C data from this project for alternative analyses are advised to use only datasets generated with the optimal cross-linking conditions of 1% Formaldehyde + 3mM DSG. The study authors further advise users to carefully select digestion enzymes and library size to fit their needs. A list of preferred datasets for re-use is provided.

Assay Description  



Experiment Set Properties  


Set Type 
Replicate
Organism 
Sample Type 
immortalized cells
Sample 
Experiment Type(s) 
Modification Type 
None
Treatment Type 
Chemical
Assay Details 
Enzyme: HindIII

5 Processed Files

Quality Metrics

Experiment
For File
Filtered Reads
Cis reads (>20kb)
Short cis reads
Trans Reads
Report
Details
From Multiple Experiments
4DNESZGE3UM2
Multiple Experiments
File
Contact List-combined (pairs)
4DNFIO8CHKOI
210.08m
72.475%
22.5%
5.026%
Experiment
4DNEX286UZDL
Bio Rep 1, Tec Rep 1
in situ Hi-C on HeLa-S3 with HindIII
File
Contact List-replicate (pairs)
4DNFIXJOFD63
210.08m
72.475%
22.5%
5.026%
Experiment
For File
Failed Balancing
Details
From Multiple Experiments
4DNESZGE3UM2
Multiple Experiments
File
Contact Matrix (mcool)
4DNFI2SNB5Z9
1000; 2000; 5000; 10000; 25000; 50000; 100000; 250000; 500000
Experiment
For File
Total Reads
Unmapped Reads
Multimapped Reads
Duplicate Reads
Walks
Minor Contigs
Details
Experiment
4DNEX286UZDL
Bio Rep 1, Tec Rep 1
in situ Hi-C on HeLa-S3 with HindIII
File
Alignments (bam)
4DNFIJKBWBI2
388.32m
12.173%
14.332%
19.392%
0.003%
0.082%