{"lab": {"title": "Yijun Ruan, JAX", "status": "current", "@type": ["Lab", "Item"], "uuid": "abd48785-b0e5-4453-be14-30d37a516bf3", "display_title": "Yijun Ruan, JAX", "@id": "/labs/yijun-ruan-lab/", "correspondence": [{"contact_email": "eWlqdW4ucnVhbkBqYXgub3Jn", "@id": "/users/d8ac229e-ec08-4411-be38-dc779520ea62/", "display_title": "Yijun Ruan"}], "pi": {"error": "no view permissions"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.abd48785-b0e5-4453-be14-30d37a516bf3"]}}, "tags": ["2018_01_public", "2018_01_internal"], "award": {"uuid": "029a2578-43dc-4343-8f41-694518cce304", "project": "4DN", "description": "NOFIC: This proposal seeks to fulfill a community need for a comprehensive, high-resolution genome-mapping platform that will enable investigation of the structural, functional and spatiotemporal organization of the human genome. Our ultimate goal is to deliver complex chromatin interaction network maps in the context of 3D genome structures from which the dynamics of individual genomic elements can be monitored and referenced. Here, we propose to develop a Nucleome Positioning System (NPS)-comprised of 1) a robust genome- wide mapping technology platform, 2) advanced computational modeling algorithms and 3) state-of-the-art nuclear imaging methods-that will allow users community-wide to uncover the regulatory functions of 3D genome organization in human cells. NPS will be based upon the established ChIA-PET method (1,2), enhanced by process optimizations-i.e., microfluidic-based miniaturization and Tn5-transposase-based library preparation-to facilitate the study of chromatin interactions mediated by protein factors across a broader range of human cell types (Aim 1, see also Mapping Technology Development Component). We will also optimize RICh-PET for the comprehensive mapping of chromatin interactions mediated by non-coding RNAs (Aim 1). The high-quality mapping data generated through these optimization efforts will be analyzed by a new computational platform (Three-Dimensional Nucleome Modeling Engine, or 3D-NOME) that makes use of hierarchical multi-scaling to model 3D genome structures (Aim 2, Data Analysis and Modeling component). We will also complement the 3D modeling with transcriptome, epigenome and SNP data associated with genetic diseases (GWAS) to provide functional annotation to structural units (Aim 2). We will continue by developing strategies to validate the nucleome geometry predicted by 3D-NOME both structurally, using new nuclear imaging technologies, and functionally, using cutting-edge genome- and epigenome-editing approaches, in both human cell lines and mouse models (Aim 3, Biological Validation Component). Finally, we will implement NPS to generate pilot 3D genome maps from a wide range of human cell lines and primary immune cells sorted from whole blood, to elucidate the spatiotemporal dynamics of human genome organization over major developmental and hematopoietic cell lineages, as well as among differentiating lymphocytes involved in the immune response (Aim 4, Data Generation Component). Together, these efforts will yield a powerful set of sophisticated, high-quality tools and mapping data for the larger research community, and will help establish the standards for future 3D/4D nucleome studies. They will also provide insights into the broad mechanisms that organize the structure and regulate the function of the human genome, as well as the specific mechanisms by which immune responses are regulated at the nuclear level.", "status": "current", "@type": ["Award", "Item"], "center_title": "NOFIC - Ruan", "name": "1U54DK107967-01", "@id": "/awards/1U54DK107967-01/", "display_title": "NUCLEOME POSITIONING SYSTEM FOR SPATIOTEMPORAL GENOME ORGANIZATION AND REGULATION", "pi": {"error": "no view permissions"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "files": [{"uuid": "983d23b3-a7ce-4fdb-8ce7-0ddd4f5a066d", "file_size": 827275080, "href": "/files-fastq/4DNFIZDLRC6P/@@download/4DNFIZDLRC6P.fastq.gz", "file_type_detailed": "reads (fastq)", "file_type": "reads", "file_classification": "raw file", "accession": "4DNFIZDLRC6P", "paired_end": "1", "@id": "/files-fastq/4DNFIZDLRC6P/", "upload_key": "983d23b3-a7ce-4fdb-8ce7-0ddd4f5a066d/4DNFIZDLRC6P.fastq.gz", "display_title": "4DNFIZDLRC6P.fastq.gz", "status": "released", "@type": ["FileFastq", "File", "Item"], "file_format": {"file_format": "fastq", "status": "released", "@id": "/file-formats/fastq/", "@type": ["FileFormat", "Item"], "display_title": "fastq", "uuid": "c13d06cf-218e-4f61-aaf0-91f226248b2c", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "related_files": [{"relationship_type": "paired with", "file": {"paired_end": "2", "display_title": "4DNFICALV8AE.fastq.gz", "file_type_detailed": "reads (fastq)", "@type": ["FileFastq", "File", "Item"], "uuid": "4300b776-8687-496e-baca-1bda751f451b", "file_type": "reads", "@id": "/files-fastq/4DNFICALV8AE/", "accession": "4DNFICALV8AE", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "quality_metric": {"url": "https://s3.amazonaws.com/elasticbeanstalk-fourfront-webprod-wfoutput/4DNFIZDLRC6P/fastqc_report.html", "Sequence length": "32-151", "status": "released", "uuid": "32d11069-fc86-42c1-80a1-0f7a92b5f538", "Total Sequences": 9810902, "display_title": "QualityMetricFastqc from 2018-02-12", "@id": "/quality-metrics-fastqc/32d11069-fc86-42c1-80a1-0f7a92b5f538/", "overall_quality_status": "PASS", "@type": ["QualityMetricFastqc", "QualityMetric", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "external_references": [], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, {"uuid": "4300b776-8687-496e-baca-1bda751f451b", "file_size": 871655473, "href": "/files-fastq/4DNFICALV8AE/@@download/4DNFICALV8AE.fastq.gz", "file_type_detailed": "reads (fastq)", "file_type": "reads", "file_classification": "raw file", "accession": "4DNFICALV8AE", "paired_end": "2", "@id": "/files-fastq/4DNFICALV8AE/", "upload_key": "4300b776-8687-496e-baca-1bda751f451b/4DNFICALV8AE.fastq.gz", "display_title": "4DNFICALV8AE.fastq.gz", "status": "released", "@type": ["FileFastq", "File", "Item"], "file_format": {"file_format": "fastq", "status": "released", "@id": "/file-formats/fastq/", "@type": ["FileFormat", "Item"], "display_title": "fastq", "uuid": "c13d06cf-218e-4f61-aaf0-91f226248b2c", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "related_files": [{"relationship_type": "paired with", "file": {"paired_end": "1", "display_title": "4DNFIZDLRC6P.fastq.gz", "file_type_detailed": "reads (fastq)", "@type": ["FileFastq", "File", "Item"], "uuid": "983d23b3-a7ce-4fdb-8ce7-0ddd4f5a066d", "file_type": "reads", "@id": "/files-fastq/4DNFIZDLRC6P/", "accession": "4DNFIZDLRC6P", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "quality_metric": {"url": "https://s3.amazonaws.com/elasticbeanstalk-fourfront-webprod-wfoutput/4DNFICALV8AE/fastqc_report.html", "Sequence length": "32-151", "status": "released", "uuid": "45f33936-0287-4ea6-95dd-bd31f165f2d8", "Total Sequences": 9810902, "display_title": "QualityMetricFastqc from 2018-02-12", "@id": "/quality-metrics-fastqc/45f33936-0287-4ea6-95dd-bd31f165f2d8/", "overall_quality_status": "PASS", "@type": ["QualityMetricFastqc", "QualityMetric", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "external_references": [], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, {"uuid": "f4ea2ebe-3463-488b-a49c-dc3923503f1d", "file_size": 13010692627, "href": "/files-fastq/4DNFIDRUF8TL/@@download/4DNFIDRUF8TL.fastq.gz", "file_type_detailed": "reads (fastq)", "file_type": "reads", "file_classification": "raw file", "accession": "4DNFIDRUF8TL", "paired_end": "1", "@id": "/files-fastq/4DNFIDRUF8TL/", "upload_key": "f4ea2ebe-3463-488b-a49c-dc3923503f1d/4DNFIDRUF8TL.fastq.gz", "display_title": "4DNFIDRUF8TL.fastq.gz", "status": "released", "@type": ["FileFastq", "File", "Item"], "file_format": {"file_format": "fastq", "status": "released", "@id": "/file-formats/fastq/", "@type": ["FileFormat", "Item"], "display_title": "fastq", "uuid": "c13d06cf-218e-4f61-aaf0-91f226248b2c", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "related_files": [{"relationship_type": "paired with", "file": {"paired_end": "2", "display_title": "4DNFI91YZTQN.fastq.gz", "file_type_detailed": "reads (fastq)", "@type": ["FileFastq", "File", "Item"], "uuid": "6921a235-9a8e-4eda-91a4-14c39eaf51c7", "file_type": "reads", "@id": "/files-fastq/4DNFI91YZTQN/", "accession": "4DNFI91YZTQN", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "quality_metric": {"url": "https://s3.amazonaws.com/elasticbeanstalk-fourfront-webprod-wfoutput/4DNFIDRUF8TL/fastqc_report.html", "Sequence length": "6-151", "status": "released", "uuid": "7c59b464-f2ae-4b8a-90c9-49e6fea603c8", "Total Sequences": 130062956, "display_title": "QualityMetricFastqc from 2018-02-12", "@id": "/quality-metrics-fastqc/7c59b464-f2ae-4b8a-90c9-49e6fea603c8/", "overall_quality_status": "PASS", "@type": ["QualityMetricFastqc", "QualityMetric", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "external_references": [], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, {"uuid": "6921a235-9a8e-4eda-91a4-14c39eaf51c7", "file_size": 14097379216, "href": "/files-fastq/4DNFI91YZTQN/@@download/4DNFI91YZTQN.fastq.gz", "file_type_detailed": "reads (fastq)", "file_type": "reads", "file_classification": "raw file", "accession": "4DNFI91YZTQN", "paired_end": "2", "@id": "/files-fastq/4DNFI91YZTQN/", "upload_key": "6921a235-9a8e-4eda-91a4-14c39eaf51c7/4DNFI91YZTQN.fastq.gz", "display_title": "4DNFI91YZTQN.fastq.gz", "status": "released", "@type": ["FileFastq", "File", "Item"], "file_format": {"file_format": "fastq", "status": "released", "@id": "/file-formats/fastq/", "@type": ["FileFormat", "Item"], "display_title": "fastq", "uuid": "c13d06cf-218e-4f61-aaf0-91f226248b2c", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "related_files": [{"relationship_type": "paired with", "file": {"paired_end": "1", "display_title": "4DNFIDRUF8TL.fastq.gz", "file_type_detailed": "reads (fastq)", "@type": ["FileFastq", "File", "Item"], "uuid": "f4ea2ebe-3463-488b-a49c-dc3923503f1d", "file_type": "reads", "@id": "/files-fastq/4DNFIDRUF8TL/", "accession": "4DNFIDRUF8TL", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "quality_metric": {"url": "https://s3.amazonaws.com/elasticbeanstalk-fourfront-webprod-wfoutput/4DNFI91YZTQN/fastqc_report.html", "Sequence length": "4-151", "status": "released", "uuid": "4b1b53ba-feaa-4dd7-980e-2e1ec709dfd1", "Total Sequences": 130062956, "display_title": "QualityMetricFastqc from 2018-02-12", "@id": "/quality-metrics-fastqc/4b1b53ba-feaa-4dd7-980e-2e1ec709dfd1/", "overall_quality_status": "PASS", "@type": ["QualityMetricFastqc", "QualityMetric", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "external_references": [], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "notes": "ENCAB871TEY; Old Target: 5d895597-c0a4-4928-b827-17c403621ed0", "status": "released", "aliases": ["ruan-lab:GM12878_CTCF_rep1_experiment"], "antibody": {"uuid": "3be6dc52-30b5-49d0-9d7a-1709435923ec", "antibody_name": "anti-CTCF", "antibody_product_no": "ab70303", "display_title": "anti-CTCF (Abcam, ab70303)", "@id": "/antibodys/anti-CTCF-ab70303/", "status": "released", "@type": ["Antibody", "Item"], "antibody_vendor": {"status": "released", "display_title": "Abcam", "@type": ["Vendor", "Item"], "uuid": "13540f71-312d-441e-a2dc-ea0dfa7e0841", "title": "Abcam", "@id": "/vendors/abcam/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "protocol": {"@id": "/protocols/f1c11c3c-0135-420d-acb5-60112dc24557/", "status": "released", "display_title": "4DN Standard Long Read ChIA-PET protocol", "uuid": "f1c11c3c-0135-420d-acb5-60112dc24557", "@type": ["Protocol", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "accession": "4DNEX6B5D93H", "biosample": {"status": "released", "@id": "/biosamples/4DNBSP88YSJR/", "display_title": "4DNBSP88YSJR", "accession": "4DNBSP88YSJR", "@type": ["Biosample", "Item"], "biosample_type": "immortalized cells", "biosource_summary": "GM12878", "uuid": "237a8a31-41f5-4c9e-9771-9e25ae227b14", "treatments_summary": "None", "modifications_summary": "None", "biosource": [{"lab": {"uuid": "828cd4fe-ebb0-4b36-a94a-d2e3a36cc989", "@type": ["Lab", "Item"], "display_title": "4DN DCIC, HMS", "status": "current", "@id": "/labs/4dn-dcic-lab/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.828cd4fe-ebb0-4b36-a94a-d2e3a36cc989"]}}, "url": "https://catalog.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=GM12878&Product=CC", "award": {"uuid": "b0b9c607-f8b4-4f02-93f4-9895b461334b", "@type": ["Award", "Item"], "status": "current", "@id": "/awards/1U01CA200059-01/", "display_title": "4D NUCLEOME NETWORK DATA COORDINATION AND INTEGRATION CENTER - PHASE I", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "status": "released", "tissue": {"term_id": "CL:0000945", "uuid": "0c98ef0b-9a03-43f3-b409-fb091e62e2f8", "status": "released", "term_name": "lymphocyte of B lineage", "@type": ["OntologyTerm", "Item"], "@id": "/ontology-terms/CL:0000945/", "display_title": "B-lymphocyte", "slim_terms": [{"uuid": "45d2b02e-130b-40db-8bf2-2288c6c57dcf", "@id": "/ontology-terms/CL:0000000/", "status": "released", "@type": ["OntologyTerm", "Item"], "display_title": "cell", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, {"uuid": "111133bc-8535-4448-903e-854af460a233", "@id": "/ontology-terms/UBERON:0002405/", "status": "released", "@type": ["OntologyTerm", "Item"], "display_title": "immune system", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, {"uuid": "72e16a19-eef3-46ca-a1b8-20e646e69675", "@id": "/ontology-terms/GO:0005623/", "status": "current", "@type": ["OntologyTerm", "Item"], "display_title": "cell", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "preferred_name": "B-lymphocyte", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "aliases": ["dcic:gm12878", "4dn-dcic-lab:gm12878"], "accession": "4DNSRVWZS4XO", "cell_line": {"slim_terms": [{"display_title": "cell", "@id": "/ontology-terms/GO:0005623/", "status": "current", "@type": ["OntologyTerm", "Item"], "uuid": "72e16a19-eef3-46ca-a1b8-20e646e69675", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, {"display_title": "cell", "@id": "/ontology-terms/CL:0000000/", "status": "released", "@type": ["OntologyTerm", "Item"], "uuid": "45d2b02e-130b-40db-8bf2-2288c6c57dcf", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "@id": "/ontology-terms/EFO:0002784/", "uuid": "b9668b9a-be39-47de-8eab-5bf1b0854417", "term_name": "GM12878", "term_id": "EFO:0002784", "@type": ["OntologyTerm", "Item"], "display_title": "GM12878", "preferred_name": "GM12878", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "individual": {"status": "released", "uuid": "3ec2ff42-6f8c-42e9-a1f0-0fa69f482b85", "@id": "/individuals-human/4DNINGWYEI15/", "protected_data": false, "display_title": "4DNINGWYEI15", "@type": ["IndividualHuman", "Individual", "Item"], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "references": [], "description": "Homo sapiens\u00a0GM12878 immortalized cell line", "date_created": "2017-04-09T17:24:19.894425+00:00", "submitted_by": {"error": "no view permissions"}, "last_modified": {"modified_by": {"error": "no view permissions"}, "date_modified": "2019-06-04T21:02:03.442329+00:00"}, "modifications": [], "biosource_type": "immortalized cell line", "cell_line_tier": "Unclassified", "public_release": "2017-07-20", "schema_version": "2", "project_release": "2017-07-20", "biosource_vendor": {"display_title": "Coriell Institute for Medical Research", "status": "released", "@type": ["Vendor", "Item"], "uuid": "f0e9a370-7451-40e3-9a35-c36d77ebdb4b", "@id": "/vendors/coriell-institute-for-medical-research/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "alternate_accessions": [], "@id": "/biosources/4DNSRVWZS4XO/", "@type": ["Biosource", "Item"], "uuid": "0f011b1e-b772-4f2a-8c24-cc55de28a994", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "display_title": "GM12878 - 4DNSRVWZS4XO", "external_references": [], "biosource_name": "GM12878", "biosource_category": ["GM12878", "1000 genomes/Hap Map"], "organism": {"display_title": "H. sapiens", "name": "human", "@id": "/organisms/9606/", "@type": ["Organism", "Item"], "status": "released", "uuid": "7745b647-ff15-4ff3-9ced-b897d4e2983c", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "badges": [{"messages": ["Biosample missing culture_harvest_date", "Biosample missing doubling_number", "Biosample missing passage_number", "Biosample missing culture_duration", "Biosample missing morphology_image"], "badge": {"@id": "/badges/biosample-metadata-incomplete/", "description": "Biosample is missing metadata information required as part of the standards implemented by the 4DN Samples working group.", "status": "released", "display_title": "Biosample Metadata Incomplete", "uuid": "2b2cc7ff-b7a8-4138-9a6c-22884fc71690", "@type": ["Badge", "Item"], "badge_icon": "/static/img/badges/biosample-icon.svg", "title": "Biosample Metadata Incomplete", "warning": "Biosample Metadata Incomplete", "badge_classification": "Warning", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}}], "modifications": [], "treatments": [], "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "description": "Replicate 1 of CTCF ChIA-PET in GM12878", "follows_sop": "Yes", "sop_mapping": {"has_sop": "No"}, "date_created": "2018-01-19T19:21:08.046439+00:00", "submitted_by": {"error": "no view permissions"}, "last_modified": {"modified_by": {"error": "no view permissions"}, "date_modified": "2019-12-03T20:41:51.534056+00:00"}, "public_release": "2018-02-23", "schema_version": "5", "experiment_type": {"display_title": "ChIA-PET", "title": "ChIA-PET", "@type": ["ExperimentType", "Item"], "uuid": "f1338cf6-8644-41e1-baaa-6691a178ac34", "experiment_category": "Sequencing", "status": "released", "assay_subclass_short": "IP-based 3C", "@id": "/experiment-types/chia-pet/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "project_release": "2018-02-23", "targeted_factor": [{"uuid": "8efeec9a-bea3-4b17-b884-4690d86fc3c7", "@type": ["BioFeature", "Item"], "status": "released", "@id": "/bio-features/8efeec9a-bea3-4b17-b884-4690d86fc3c7/", "display_title": "CTCF protein", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "biosample_quantity": 200000000, "other_processed_files": [{"type": "preliminary", "files": [{"display_title": "4DNFIIDY7MGH.bam", "file_type": "alignments", "@type": ["FileProcessed", "File", "Item"], "uuid": "7e7f3ed5-87b4-4901-9eb8-6fbce564f556", "file_type_detailed": "alignments (bam)", "status": "released", "href": "/files-processed/4DNFIIDY7MGH/@@download/4DNFIIDY7MGH.bam", "@id": "/files-processed/4DNFIIDY7MGH/", "genome_assembly": "GRCh38", "file_size": 30722602934, "accession": "4DNFIIDY7MGH", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/wfoutput/7e7f3ed5-87b4-4901-9eb8-6fbce564f556/4DNFIIDY7MGH.bam", "quality_metric": {"@type": ["QualityMetricBamqc", "QualityMetric", "Item"], "status": "released", "overall_quality_status": "PASS", "display_title": "QualityMetricBamqc from 2019-12-20", "uuid": "cae88bb3-129a-4544-a294-8f00d345d13e", "@id": "/quality-metrics-bamqc/cae88bb3-129a-4544-a294-8f00d345d13e/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "quality_metric_summary": [{"title": "Total Reads", "value": "139873858", "numberType": "integer"}, {"title": "Unmapped Reads", "value": "7.677", "tooltip": "Percent of total reads (=10.74m)", "numberType": "percent"}, {"title": "Multimapped Reads", "value": "6.088", "tooltip": "Percent of total reads (=8.52m)", "numberType": "percent"}, {"title": "Duplicate Reads", "value": "18.126", "tooltip": "Percent of total reads (=25.35m)", "numberType": "percent"}, {"title": "Walks", "value": "4.825", "tooltip": "Percent of total reads (=6.75m)", "numberType": "percent"}, {"title": "Minor Contigs", "value": "0.293", "tooltip": "Percent of total reads (=0.41m)", "numberType": "percent"}]}, "track_and_facet_info": {"experimental_lab": "Yijun Ruan, JAX", "experiment_type": "ChIA-PET", "experiment_bucket": "HiC Processing Pipeline Results on ChIA-PET data", "assay_info": "CTCF protein", "dataset": "ChIA-PET in GM12878", "condition": "CTCF", "replicate_info": "Biorep 1, Techrep 1", "biosource_name": "GM12878", "lab_name": "4DN DCIC, HMS"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "file_format": {"@type": ["FileFormat", "Item"], "display_title": "bam", "uuid": "d13d06cf-218e-4f61-aaf0-91f226248b3c", "file_format": "bam", "@id": "/file-formats/bam/", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "last_modified": {"date_modified": "2021-03-28T16:21:00.392285+00:00"}}, {"display_title": "4DNFIHT2VYC1.pairs.gz", "file_type": "contact list-replicate", "@type": ["FileProcessed", "File", "Item"], "uuid": "d95d6276-64ba-47c6-ad37-0efda9368dcc", "file_type_detailed": "contact list-replicate (pairs)", "status": "released", "href": "/files-processed/4DNFIHT2VYC1/@@download/4DNFIHT2VYC1.pairs.gz", "@id": "/files-processed/4DNFIHT2VYC1/", "genome_assembly": "GRCh38", "file_size": 1510678028, "accession": "4DNFIHT2VYC1", "open_data_url": "https://4dn-open-data-public.s3.amazonaws.com/fourfront-webprod/wfoutput/d95d6276-64ba-47c6-ad37-0efda9368dcc/4DNFIHT2VYC1.pairs.gz", "quality_metric": {"@type": ["QualityMetricPairsqc", "QualityMetric", "Item"], "status": "released", "overall_quality_status": "PASS", "display_title": "QualityMetricPairsqc from 2018-06-07", "uuid": "77858509-0042-40a3-a83e-821ba0f2cdd5", "url": "https://s3.amazonaws.com/elasticbeanstalk-fourfront-webprod-wfoutput/4DNFIHT2VYC1/pairsqc_report.html", "@id": "/quality-metrics-pairsqc/77858509-0042-40a3-a83e-821ba0f2cdd5/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "quality_metric_summary": [{"title": "Filtered Reads", "value": "88518417", "numberType": "integer"}, {"title": "Cis reads (>20kb)", "value": "2.56", "tooltip": "Percent of filtered reads (=2.27m)", "numberType": "percent"}, {"title": "Short cis reads", "value": "87.606", "tooltip": "Percent of filtered reads (=77.55m)", "numberType": "percent"}, {"title": "Trans Reads", "value": "9.835", "tooltip": "Percent of filtered reads (=8.71m)", "numberType": "percent"}]}, "track_and_facet_info": {"experimental_lab": "Yijun Ruan, JAX", "experiment_type": "ChIA-PET", "experiment_bucket": "HiC Processing Pipeline Results on ChIA-PET data", "assay_info": "CTCF protein", "dataset": "ChIA-PET in GM12878", "condition": "CTCF", "replicate_info": "Biorep 1, Techrep 1", "biosource_name": "GM12878", "lab_name": "4DN DCIC, HMS"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "file_format": {"@type": ["FileFormat", "Item"], "display_title": "pairs", "uuid": "d13d06cf-218e-4f61-aaf0-91f226248b2c", "file_format": "pairs", "@id": "/file-formats/pairs/", "status": "released", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "last_modified": {"date_modified": "2021-12-16T16:56:49.269148+00:00"}}], "title": "HiC Processing Pipeline Results on ChIA-PET data", "description": "These results are provided for comparison. The processing may not be optimal since the pipeline does not take into account DNA linkers used in ChIA-PET experiments."}], "biosample_quantity_units": "cells", "library_preparation_date": "2014-04-21", "@id": "/experiments-chiapet/4DNEX6B5D93H/", "@type": ["ExperimentChiapet", "Experiment", "Item"], "uuid": "7fed14cc-2464-45bd-a387-7a25f3779f35", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "display_title": "ChIA-PET against CTCF protein on GM12878 - 4DNEX6B5D93H", "external_references": [], "experiment_sets": [{"experimentset_type": "replicate", "status": "released", "@id": "/experiment-set-replicates/4DNESNPHX8LY/", "accession": "4DNESNPHX8LY", "uuid": "069d5b42-b235-4bc0-a80d-fe9e6c6dc79a", "@type": ["ExperimentSetReplicate", "ExperimentSet", "Item"], "display_title": "4DNESNPHX8LY", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "publications_of_exp": [], "experiment_categorizer": {"field": "Target", "value": "CTCF protein", "combined": "Target: CTCF protein"}, "experiment_summary": "ChIA-PET against CTCF protein on GM12878", "@context": "/terms/", "aggregated-items": {"badges": [{"parent": "/biosamples/4DNBSP88YSJR/", "embedded_path": "biosample.badges", "item": {"messages": ["Biosample missing culture_harvest_date", "Biosample missing doubling_number", "Biosample missing passage_number", "Biosample missing culture_duration", "Biosample missing morphology_image"], "badge": {"commendation": null, "warning": "Biosample Metadata Incomplete", "uuid": "2b2cc7ff-b7a8-4138-9a6c-22884fc71690", "@id": "/badges/biosample-metadata-incomplete/", "badge_icon": "/static/img/badges/biosample-icon.svg", "description": "Biosample is missing metadata information required as part of the standards implemented by the 4DN Samples working group."}}}]}, "validation-errors": []}