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{"view": ["system.Everyone"], "edit": ["group.admin"]}}], "produced_in_pub": {"@type": ["Publication", "Item"], "ID": "PMID:29313530", "date_published": "2018-02", "abstract": "Long-range chromatin interactions between enhancers and promoters are essential for transcription of many developmentally controlled genes in mammals and other metazoans. Currently, the exact mechanisms that connect distal enhancers to their specific target promoters remain to be fully elucidated. Here, we show that the enhancer-specific histone H3 lysine 4 monomethylation (H3K4me1) and the histone methyltransferases MLL3 and MLL4 (MLL3/4) play an active role in this process. We demonstrate that in differentiating mouse embryonic stem cells, MLL3/4-dependent  deposition of H3K4me1 at enhancers correlates with increased levels of chromatin  interactions, whereas loss of this histone modification leads to reduced levels of chromatin interactions and defects in gene activation during differentiation.  H3K4me1 facilitates recruitment of the Cohesin complex, a known regulator of chromatin organization, to chromatin in vitro and in vivo, providing a potential  mechanism for MLL3/4 to promote chromatin interactions between enhancers and promoters. Taken together, our results support a role for MLL3/4-dependent H3K4me1 in orchestrating long-range chromatin interactions at enhancers in mammalian cells.", "title": "Histone H3 lysine 4 monomethylation modulates long-range chromatin interactions at enhancers.", "short_attribution": "Yan J et al. (2018)", "status": "current", "authors": ["Yan J", "Chen SA", "Local A", "Liu T", "Qiu Y", "Dorighi KM", "Preissl S", "Rivera CM", "Wang C", "Ye Z", "Ge K", "Hu M", "Wysocka J", "Ren B"], "uuid": "daea24a9-b600-40f2-aa43-dae13b63bffb", "display_title": "Yan J et al. 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Here, we show that the enhancer-specific histone H3 lysine 4 monomethylation (H3K4me1) and the histone methyltransferases MLL3 and MLL4 (MLL3/4) play an active role in this process. We demonstrate that in differentiating mouse embryonic stem cells, MLL3/4-dependent  deposition of H3K4me1 at enhancers correlates with increased levels of chromatin  interactions, whereas loss of this histone modification leads to reduced levels of chromatin interactions and defects in gene activation during differentiation.  H3K4me1 facilitates recruitment of the Cohesin complex, a known regulator of chromatin organization, to chromatin in vitro and in vivo, providing a potential  mechanism for MLL3/4 to promote chromatin interactions between enhancers and promoters. Taken together, our results support a role for MLL3/4-dependent H3K4me1 in orchestrating long-range chromatin interactions at enhancers in mammalian cells.", "journal": "Cell research", "uuid": "daea24a9-b600-40f2-aa43-dae13b63bffb", "@type": ["Publication", "Item"], "ID": "PMID:29313530", "status": "current", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "experiment_categorizer": {"field": "Enzyme", "value": "MboI", "combined": "Enzyme: MboI"}, "experiment_summary": "in situ Hi-C on ES-E14 with MboI", "@context": "/terms/", "aggregated-items": {"badges": [{"parent": "/biosamples/4DNBSMX7YM2H/", "embedded_path": "biosample.badges", "item": {"messages": ["Biosample missing doubling_number", "Biosample missing passage_number", "Biosample missing morphology_image", "Biosample is a stem cell line with unknown passage number missing karyotype"], "badge": {"commendation": null, "warning": "Biosample Metadata Incomplete", "uuid": "2b2cc7ff-b7a8-4138-9a6c-22884fc71690", "@id": "/badges/biosample-metadata-incomplete/", "badge_icon": "/static/img/badges/biosample-icon.svg", "description": "Biosample is missing metadata information required as part of the standards implemented by the 4DN Samples working group."}}}]}, "validation-errors": []}