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Acute depletion of  condensin I or II shows that nested loops form by differential action of the two  condensins, whereas condensin II is required for helical winding.", "status": "current", "@id": "/publications/84fa5633-aac7-4cb3-b53b-0f0234ea06dd/", "title": "A pathway for mitotic chromosome formation.", "display_title": "Gibcus JH et al. (2018) PMID:29348367", "ID": "PMID:29348367", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "publications_of_exp": [{"ID": "PMID:29348367", "@type": ["Publication", "Item"], "status": "current", "@id": "/publications/84fa5633-aac7-4cb3-b53b-0f0234ea06dd/", "abstract": "Mitotic chromosomes fold as compact arrays of chromatin loops. To identify the pathway of mitotic chromosome formation, we combined imaging and Hi-C analysis of synchronous DT40 cell cultures with polymer simulations. Here we show that in prophase, the interphase organization is rapidly lost in a condensin-dependent manner, and arrays of consecutive 60-kilobase (kb) loops are formed. During prometaphase, ~80-kb inner loops are nested within ~400-kb outer loops. The loop  array acquires a helical arrangement with consecutive loops emanating from a central \"spiral staircase\" condensin scaffold. The size of helical turns progressively increases to ~12 megabases during prometaphase. Acute depletion of  condensin I or II shows that nested loops form by differential action of the two  condensins, whereas condensin II is required for helical winding.", "date_published": "2018-02-09", "title": "A pathway for mitotic chromosome formation.", "short_attribution": "Gibcus JH et al. (2018)", "display_title": "Gibcus JH et al. 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