ExperimentMic multiplexed FISH on A549

multiplexed FISH4DNEX46ABFUV
released
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   September 2nd, 2022 at 10:48am
Source Publication
Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells.
Bintu B, Mateo LJ, et al., Science (New York, N.Y.) 2018

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBST1RIXZA
Biosample Biosource 
A549 - 4DNSRFLY6T15
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Highly Inclined Laminated Optical Sheet
Microscope Quality Control 
None
Microscope Configuration 
HARVARD-Zhuang_lab_CT-STORM6_Nikon_Eclipse_Ti-U_Scope1
Channel
Imaging Rounds
Path
 
ch03
1-41
65 consecutive 30-kb loci on chr21 targeted by oligo probe library (with Alexa Fluor 647-labeled readout oligos)
ch03
42-65
65 consecutive 30-kb loci on chr21 targeted by oligo probe library, readout oligo library (with Alexa Fluor 647-labeled imaging oligos)
ch02
-
540/560nm fiducial beads

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