ExperimentMic Immunofluorescence on U2OS (Tier 2)

Immunofluorescence4DNEX76VDXUJ
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Validation experiment of high-throughput 3D, two-color single-molecule switching nanoscopy. This experiment tested whether the fast speeds attained by our imaging platform were sustainable for over 24 hours, and additionally served as a test of large-scale SMS nanoscopy image analysis.

   August 5th, 2019 at 3:32pm
Source Publication
An integrated platform for high-throughput nanoscopy.
Barentine AES, Lin Y, et al., Nature biotechnology 2023

Properties  

Experiment Type 
Immunofluorescence
Follows SOP 
No
Biosample 
4DNBSG6G1IE1
Biosample Biosource 
U2OS (Tier 2) - 4DNSR3V9ST6H
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
STORM
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Path
 
ch00
NPM1 protein targeted by anti-nucleophosmin (with anti-Mouse, Alexa Fluor 647)
642nm
ch01
LMNB1 protein targeted by anti-LaminB1 (with anti-Rabbit, CF568)
560nm

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