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In Drosophila the role of  insulators in 3D genome organization is, however, under current debate. Here, we  addressed this question by combining bioinformatics analysis and multiplexed  chromatin imaging. We describe a class of Drosophila insulators enriched at  regions forming preferential chromatin interactions genome-wide. Notably, most of  these 3D interactions do not involve TAD borders. Multiplexed imaging shows that  these interactions occur infrequently, and only rarely involve multiple genomic  regions coalescing together in space in single cells. Finally, we show that  non-border preferential 3D interactions enriched in this class of insulators are  present before TADs and transcription during Drosophila development. Our results  are inconsistent with insulators forming stable hubs in single cells, and instead  suggest that they fine-tune existing 3D chromatin interactions, providing an  additional regulatory layer for transcriptional regulation.", "ID": "PMID:37865700", "uuid": "52f8c166-d604-47c0-8590-b44c787a89fe", "title": "3D chromatin interactions involving Drosophila insulators are infrequent but  preferential and arise before TADs and transcription.", "display_title": "Messina O et al. (2023) PMID:37865700", "@type": ["Publication", "Item"], "short_attribution": "Messina O et al. 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Multiplexed imaging shows that  these interactions occur infrequently, and only rarely involve multiple genomic  regions coalescing together in space in single cells. Finally, we show that  non-border preferential 3D interactions enriched in this class of insulators are  present before TADs and transcription during Drosophila development. Our results  are inconsistent with insulators forming stable hubs in single cells, and instead  suggest that they fine-tune existing 3D chromatin interactions, providing an  additional regulatory layer for transcriptional regulation.", "short_attribution": "Messina O et al. (2023)", "display_title": "Messina O et al. 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