ExperimentMic multiplexed FISH on embryo

multiplexed FISH4DNEXLSGBFMV
released
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   May 20th, 2022 at 8:51am
Source Publication
Visualizing DNA folding and RNA in embryos at single-cell resolution.
Mateo LJ, Murphy SE, et al., Nature 2019

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSXDQ9QTL
Biosample Biosource 
embryo - 4DNSR13C9VV1
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Highly Inclined Laminated Optical Sheet
Microscope Quality Control 
None
Microscope Configuration 
Stanford-Boettiger_lab_ORCA_Nikon_Eclipse_Ti-U_Scope1
Channel
Path
 
ch00
70 consecutive 10-kb loci in the BX-C region (fruit fly) targeted by oligo probe library (with Cy3-labeled fiducial oligo probes)
ch01
70 consecutive 10-kb loci in the BX-C region (fruit fly) targeted by oligo probe library, readout oligo library (with Cy5-labeled imaging oligos)
ch01
29 mRNAs and nascent RNAs (fruit fly) targeted by oligo probe library, readout oligo library (with Cy5-labeled imaging oligos)

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