ExperimentMic multiplexed FISH on ES-E14TG2a with Rad21-AID

multiplexed FISH4DNEXPQST1SA
released
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   March 20th, 2023 at 10:24pm
Source Publication
Loop stacking organizes genome folding from TADs to chromosomes.
Hafner A, Park M, et al., Molecular cell 2023

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSA2IZFTU
Biosample Biosource 
ES-E14TG2a with Rad21-AID - 4DNSRF3QONOW
Biosample Modifications 
None
Biosample Treatments 
IAA treatment (500.0 μM, 4.0h)
Microscopy Technique 
Highly Inclined Laminated Optical Sheet
Microscope Quality Control 
None
Microscope Configuration 
Stanford-Boettiger_lab_ORCA_Nikon_Eclipse_Ti2-U_Scope2
Channel
Path
 
ch03
3-Mb chr6 (mouse) targeted by oligo probe library, readout oligo library (with Alexa Fluor 750-labeled imaging oligos)
ch01
3-Mb chr6 (mouse) targeted by oligo probe library (with Cy3-labeled fiducial oligo probes)
ch02
Geminin (mouse) targeted by anti-geminin (with anti-Rabbit, Alexa Fluor 647)
ch00
Chromosomes targeted by DAPI

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