ExperimentMic multiplexed FISH on H7-hESC

multiplexed FISH4DNEXVPP1CE2
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Chromatin tracing on H7 day 6 in 5iLA culture condition combined with XIST RNA FISH (XIST- chromosomes)

   August 22nd, 2023 at 7:25pm
Source Publication
Female naive human pluripotent stem cells carry X chromosomes with Xa-like and Xi-like folding conformations.
Patterson B, Yang B, et al., Science advances 2023

Properties  

Experiment Type 
multiplexed FISH
Follows SOP 
No
Biosample 
4DNBSGXJ3IAV
Biosample Biosource 
H7-hESC - 4DNSRJBQJC94
Biosample Modifications 
None
Biosample Treatments 
None
Microscopy Technique 
Widefield
Microscope Quality Control 
None
Microscope Configuration 
None
Channel
Imaging Rounds
Path
 
ch00
1-20
Alexa Fluor 488-labeled fiducial beads
ch02
1-20
20 TADs chrX (1-20) targeted by Alexa Fluor 647-labeled imaging oligos
ch03
1-20
20 TADs chrX (21-40) targeted by ATTO 565-labeled imaging oligos
ch01
-
XIST transcript targeted by Cy3-labeled imaging oligos

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