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(2025)", "title": "Reorganization of the heterochromatin-associated gene-dense subcompartment in  early neuronal development.", "journal": "Biology open", "display_title": "Scrutton Alvarado NJ et al. (2025) PMID:40353744", "ID": "PMID:40353744", "date_published": "2025-05-15", "abstract": "The 3D organization of the genome has emerged as an important regulator of  cellular development. Post-mitotic neurons undergo conserved changes in genome  organization, such as the inward radial repositioning of heterochromatin-rich  chromosomes as they differentiate. Additionally, transcriptionally active but  heterochromatin-associated gene-dense (hGD) regions significantly strengthen  their long-distance interactions during cerebellar development. However, the  specific developmental stages during which these nuclear changes take place have  remained poorly defined. Here, we report that hGD regions relocalize toward the  nuclear interior and strengthen their chromosomal interactions as immature  granule neurons transition from active cell migration to subsequent stages of  neuronal differentiation. During this period, hGD genomic regions are  coordinately repositioned in the nucleus alongside their physically tethered  heterochromatic chromocenters. Despite these major changes in nuclear  organization, the hGD subcompartment remains distinct from other  transcriptionally active or repressive nuclear bodies, including heterochromatic  chromocenters, throughout development. Notably, these nuclear changes appear to  be independent of transcriptional changes that occur during granule neuron  differentiation. Together, our results provide insights into the developmental  timing of structural changes in the chromosomes of post-mitotic neurons.", "authors": ["Scrutton Alvarado NJ", "Zhao Z", "Yamada T", "Yang Y"], "@type": ["Publication", "Item"], "status": "current", "uuid": "6e38470d-c0e1-4824-ba6b-ce5b905f94e3", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "publications_of_exp": [{"uuid": "6e38470d-c0e1-4824-ba6b-ce5b905f94e3", "@type": ["Publication", "Item"], "ID": "PMID:40353744", "abstract": "The 3D organization of the genome has emerged as an important regulator of  cellular development. Post-mitotic neurons undergo conserved changes in genome  organization, such as the inward radial repositioning of heterochromatin-rich  chromosomes as they differentiate. Additionally, transcriptionally active but  heterochromatin-associated gene-dense (hGD) regions significantly strengthen  their long-distance interactions during cerebellar development. However, the  specific developmental stages during which these nuclear changes take place have  remained poorly defined. Here, we report that hGD regions relocalize toward the  nuclear interior and strengthen their chromosomal interactions as immature  granule neurons transition from active cell migration to subsequent stages of  neuronal differentiation. During this period, hGD genomic regions are  coordinately repositioned in the nucleus alongside their physically tethered  heterochromatic chromocenters. Despite these major changes in nuclear  organization, the hGD subcompartment remains distinct from other  transcriptionally active or repressive nuclear bodies, including heterochromatic  chromocenters, throughout development. Notably, these nuclear changes appear to  be independent of transcriptional changes that occur during granule neuron  differentiation. Together, our results provide insights into the developmental  timing of structural changes in the chromosomes of post-mitotic neurons.", "@id": "/publications/6e38470d-c0e1-4824-ba6b-ce5b905f94e3/", "journal": "Biology open", "date_published": "2025-05-15", "status": "current", "title": "Reorganization of the heterochromatin-associated gene-dense subcompartment in  early neuronal development.", "short_attribution": "Scrutton Alvarado NJ et al. (2025)", "authors": ["Scrutton Alvarado NJ", "Zhao Z", "Yamada T", "Yang Y"], "display_title": "Scrutton Alvarado NJ et al. (2025) PMID:40353744", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}], "experiment_categorizer": {"field": "Default", "value": null}, "experiment_summary": "RNA-seq on mitotic cerebellar granule neuron precursor - PND6", "@context": "/terms/", "aggregated-items": {"badges": []}, "validation-errors": []}