ImagingPath
65 consecutive 30-kb loci in a region without TADs on chr21 targeted by oligo probe library, readout oligo library (with Alexa Fluor 647-labeled imaging oligos)

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Sequential DNA FISH of 24 consecutive 30-kb loci (rounds # 42-65) in a 2-Mb region lacking TAD boundaries on chromosome 21 (Chr21:18.6Mb-20.6Mb) by sequential hybridization of readout oligos complementary to primary oligo probes and labelled with AF647-conjugated imaging oligos

   September 2nd, 2022 at 10:48am

Details

experiment_type 
DNA FISH
aliases 
  1. alistair-boettiger-lab:path_2-Mb_no_TAD_42-65
contributing_labs 
  1. Xiaowei Zhuang, HARVARD
file_reference 
  1. 4DNFIFA8KDUE.bed.gz
  2. 4DNFILBDMLUL.zip
imaging_rounds 
42-65
labeled_probe 
imaging oligos
labels 
  1. Alexa Fluor 647
other_probes 
  1. oligo probe library
  2. readout oligo library
public_release 
October 20th, 2022
target 
  1. 65 consecutive 30-kb loci in a region without TADs on chr21
type_labeled_probe 
DNA primer