{"ID": "PMID:32105612", "lab": {"display_title": "Ahilya Sawh Lab", "correspondence": [{"contact_email": "YWhpbHlhLnNhd2hAZ21haWwuY29t", "@id": "/users/a3b6e5d9-34ea-4997-a4cb-e4204c90bac5/", "display_title": "Ahilya Sawh"}], "@type": ["Lab", "Item"], "uuid": "fe7249ca-3622-469a-ad9d-e5a85441bf52", "title": "Ahilya Sawh Lab", "status": "current", "@id": "/labs/ahilya-sawh-lab/", "pi": {"error": "no view permissions"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.fe7249ca-3622-469a-ad9d-e5a85441bf52"]}}, "url": "https://www.ncbi.nlm.nih.gov/pubmed/32105612", "award": {"uuid": "12a92962-8265-4fc0-b2f8-cf14f05db58b", "@type": ["Award", "Item"], "description": "Funding source is from outside 4DN.", "center_title": "External", "project": "External", "center": "External", "name": "external-award", "display_title": "EXTERNAL AWARD", "status": "current", "@id": "/awards/external-award/", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "title": "Lamina-Dependent Stretching and Unconventional Chromosome Compartments in Early  C. elegans Embryos.", "status": "current", "aliases": ["external-lab:c_elegans_multiplexed-fish"], "authors": ["Sawh AN", "Shafer MER", "Su JH", "Zhuang X", "Wang S", "Mango SE"], "journal": "Molecular cell", "abstract": "Current models suggest that chromosome domains segregate into either an active  (A) or inactive (B) compartment. B-compartment chromatin is physically separated  from the A compartment and compacted by the nuclear lamina. To examine these  models in the developmental context of C. elegans embryogenesis, we undertook  chromosome tracing to map the trajectories of entire autosomes. Early embryonic  chromosomes organized into an unconventional barbell-like configuration, with two  densely folded B compartments separated by a central A compartment. Upon  gastrulation, this conformation matured into conventional A/B compartments. We  used unsupervised clustering to uncover subpopulations with differing folding  properties and variable positioning of compartment boundaries. These  conformations relied on tethering to the lamina to stretch the chromosome;  detachment from the lamina compacted, and allowed intermingling between, A/B  compartments. These findings reveal the diverse conformations of early embryonic  chromosomes and uncover a previously unappreciated role for the lamina in  systemic chromosome stretching.", "date_created": "2025-04-02T20:47:29.983848+00:00", "submitted_by": {"error": "no view permissions"}, "last_modified": {"modified_by": {"error": "no view permissions"}, "date_modified": "2025-04-08T19:23:58.733131+00:00"}, "date_published": "2020-04-02", "public_release": "2025-04-08", "schema_version": "2", "project_release": "2025-04-08", "contributing_labs": [{"uuid": "9827adc7-c9a9-44ae-a689-c83e09fee179", "correspondence": [{"contact_email": "c3VzYW4ubWFuZ29AdW5pYmFzLmNo", "@id": "/users/8b7b64e6-0e32-4635-8d23-568b29ddda6a/", "display_title": "Susan Mango"}], "@id": "/labs/susan-mango-lab/", "status": "current", "@type": ["Lab", "Item"], "display_title": "Susan Mango", "pi": {"error": "no view permissions"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.9827adc7-c9a9-44ae-a689-c83e09fee179"]}}], "exp_sets_prod_in_pub": [{"accession": "4DNESO52B8EA", "@id": "/experiment-set-replicates/4DNESO52B8EA/", "display_title": "4DNESO52B8EA", "uuid": "1ad0f794-a617-49f2-bfd6-85fd55076207", "experimentset_type": "replicate", "@type": ["ExperimentSetReplicate", "ExperimentSet", "Item"], "status": "released", "experiments_in_set": [{"uuid": "a6cedce0-9199-45aa-b21e-ff51e6e3af76", "display_title": "multiplexed FISH on multicellular organism - 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