{"ID": "doi:10.1016/j.cell.2016.11.054", "lab": {"@type": ["Lab", "Item"], "uuid": "eff9a919-3735-4a9d-b5f5-6eac396f2d6f", "display_title": "Cliff Brangwynne, PRINCETON", "title": "Cliff Brangwynne, PRINCETON", "correspondence": [{"contact_email": "Y2JyYW5nd3lAcHJpbmNldG9uLmVkdQ==", "@id": "/users/59b327ee-16cf-4f28-b7cd-eda7f63def6b/", "display_title": "Cliff Brangwynne"}], "@id": "/labs/cliff-brangwynne-lab/", "status": "current", "pi": {"error": "no view permissions"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin", "role.lab_submitter", "submits_for.eff9a919-3735-4a9d-b5f5-6eac396f2d6f"]}}, "url": "https://www.ncbi.nlm.nih.gov/pubmed/28041848", "award": {"name": "1U01DA040601-01", "status": "current", "description": "NBC: Nuclear bodies (NBs) are ubiquitous membrane-less structures that play important but poorly-understood roles in gene regulation. NBs locally increase the concentration of molecules involved in chromatin remodeling, transcription initiation, and RNA processing. Despite their functional importance, and decades of study, we lack a quantitative, mechanistic understanding of NB assembly. Understanding the biophysical rules governing NB assembly and properties is key to elucidating their function. Our group has pioneered the concept that NBs are liquid phase droplets that assemble through phase transitions. Here we will build on this framework, and test it, by developing a new technology that uses light to control nucleoplasmic phase transitions. This technology will enable precise spatiotemporal control of the assembly of NBs and their viscoelastic properties, as well as testing the impact on composition, function, and genome architecture. Our team is uniquely positioned to develop this exciting technology and exploit it to study NBs, both in these Specific Aims, as well as together in future collaborations within the 4D Nucleome Program.", "display_title": "OPTOGENETIC DROPLETS: USING LIGHT TO CONTROL NUCLEOPLASMIC PHASE SEPARATION", "uuid": "2a3fa7b0-4e64-411a-87e0-2b57670bfa23", "center_title": "NBC - Brangwynne", "@id": "/awards/1U01DA040601-01/", "project": "4DN", "@type": ["Award", "Item"], "pi": {"error": "no view permissions"}, "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}}, "title": "Spatiotemporal Control of Intracellular Phase Transitions Using Light-Activated optoDroplets.", "status": "current", "aliases": ["4dn-dcic-lab:optodroplet_pub"], "authors": ["Shin Y", "Berry J", "Pannucci N", "Haataja MP", "Toettcher JE", "Brangwynne CP"], "journal": "Cell", "abstract": "Phase transitions driven by intrinsically disordered protein regions (IDRs) have  emerged as a ubiquitous mechanism for assembling liquid-like RNA/protein (RNP) bodies and other membrane-less organelles. However, a lack of tools to control intracellular phase transitions limits our ability to understand their role in cell physiology and disease. Here, we introduce an optogenetic platform that uses light to activate IDR-mediated phase transitions in living cells. We use this \"optoDroplet\" system to study condensed phases driven by the IDRs of various RNP  body proteins, including FUS, DDX4, and HNRNPA1. Above a concentration threshold, these constructs undergo light-activated phase separation, forming spatiotemporally definable liquid optoDroplets. FUS optoDroplet assembly is fully reversible even after multiple activation cycles. However, cells driven deep within the phase boundary form solid-like gels that undergo aging into irreversible aggregates. This system can thus elucidate not only physiological phase transitions but also their link to pathological aggregates.", "categories": ["methods paper"], "date_created": "2020-04-14T15:07:30.000494+00:00", "submitted_by": {"error": "no view permissions"}, "last_modified": {"modified_by": {"error": "no view permissions"}, "date_modified": "2020-04-14T15:40:22.792883+00:00"}, "date_published": "2017-01-12", "public_release": "2020-04-14", "schema_version": "2", "project_release": "2020-04-14", "@id": "/publications/fb7aee70-4a6d-4c25-b9e9-f1dc21779c3f/", "@type": ["Publication", "Item"], "uuid": "fb7aee70-4a6d-4c25-b9e9-f1dc21779c3f", "principals_allowed": {"view": ["system.Everyone"], "edit": ["group.admin"]}, "display_title": "Shin Y et al. (2017) doi:10.1016/j.cell.2016.11.054", "external_references": [], "short_attribution": "Shin Y et al. (2017)", "@context": "/terms/", "aggregated-items": {}, "validation-errors": []}